Coding

Part:BBa_K3255000:Design

Designed by: Igor Martinez, Juan Nogales   Group: iGEM19_MADRID_UCM_HS   (2019-10-16)


Enzyme of the athocyanine route synthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 47
    Illegal PstI site found at 359
    Illegal PstI site found at 704
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

. The gene has been cloned via Sma I in SEVA181 vector. It has been designed to be digested with Bsa I, it has ampicillin resistance and pUC origin.

Source

The coding gene has a plant origin, from Camelia sinensis

References

. J. A. Jones, V. R. Vernacchio, S. M. Collins, A. N. Shirk, Y. Xiu, J. A. Englaender, B. F. Cress, C. C. McCutcheon, R. J. Linhardt, R. A. Gross, M. A. G. Koffas. Complete Biosynthesis of Anthocyanins Using E. coli Polycultures. mBio 8 (2017) e00621-17.

J. Zha, M. A.G. Koffas. Production of anthocyanins in metabolically engineered microorganisms: Current status and perspectives. Synthetic and Systems Biotechnology 2 (2017) 259-266.

I. Appelhagen, A. K. Wulff-Vester, M. Wendell, A.-K. Hvoslef-Eide, J. Russell, A. Oertel, S. Martens, H.-P. Mock, C. Martin, A. Matros. Colour bio-factories: Towards scale-up production of anthocyanins in plant cell cultures. Metabolic Engineering 48 (2018) 218-232.

B. Shrestha, R. P. Pandey, S. Darsandhari, P. Parajuli, J. K. Sohng. Combinatorial approach for improved cyanidin 3‑O‑glucoside production in Escherichia coli. Microb Cell Fact 18 (2019) 7.